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KMID : 1101420160480020124
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Korean Journal of Clinical Laboratory Science
2016 Volume.48 No. 2 p.124 ~ p.129
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Comparison of Quantitative Endotoxin against 5 Species of Enterobacteriaceae
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Kwon Pil-Seung
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Abstract
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Endotoxin, also known as lipopolysaccharide (LPS) produced by the cell wall of gram negative bacteria can be present in any liquid or on any biomaterial. Endotoxin in blood can cause fever and inflammation. In this study, we compared bacterial endotoxin using Escherichia coli O157:H7, Klebsiella oxytoca, Salmonella Typhi, Shigella sonnei and Morganella morganii. Bacteria were cultured for use in the experiment, and diluted to 1.5×108 CFU/mL. A check marked sensitivity confirmatory test of the Limulus amebocyte lysate (LAL) reagent was performed to examine the validity. The end point reaction to each bacteria sample was confirmed with 10 fold dilution and then the final reaction end point was confirmed by 2 fold dilution between the dilution step and the upper dilution step. According to the results, in detection of endotoxins in more than 0.015 EU/mL, E. coli O157 was 75∼37.5 CFU/mL, K. oxytoca 37.5∼18.75 CFU/mL, M. morganii and S. Typhi 3.75∼1.875 CFU/mL, and S. sonnei 7.5∼3.75 CFU/mL. The resulting value was finally ensured by a confirmation test for the inhibitory factor. Based on this study, conduct of further research on bacterial endotoxin is encouraged.
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KEYWORD
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Endotoxin, Lipopolysaccharide, Limulus amebocyte lysate, Enterobacteriaceae
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